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RNP-containing basal cells were then expanded on rat collagen-coated dishes in PEP medium with RI, and seeded onto transwell inserts (1 10 5 cells/insert) in PEP medium ( FOXN4 KO) or ALI expansion medium ( FOXI1 and POU2F3 KOs) with RI

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Abnormal activation of TLRs leads to an exaggerated autoimmune response with increased production of cytokines such as IL17A and IL22, which are responsible for hyperproliferation and abnormal differentiation of keratinocytes, ultimately leading to the formation of psoriatic plaques (Sun et al., 2019)