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The model showed R2 and Q2 values of 0.99 indicating a good classification performance and validated using permutation test with 20 times and CV-ANOVA which demonstrated that the model is not over fitted (Supplementary Fig

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Binding buffer conditions are optimized to ensure that the target molecules interact effectively with the ligand and are retained by the affinity medium as all other molecules wash through the column

Such damage can further lead to mitochondrial dysfunction, triggering apoptosis or necroptosis, which represents a key pathological process in oxidative stress [61]