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269 While ROSALIND is limited to the use of well-characterized apo-repressors, cell extract-based In vitro transcription-translation (Tx-Tl) systems permit the development of biosensors with the whole array of possible aTFs 622,651,652 and orthogonal regulatory elements, including riboswitches (Section 3.4), 653 since both protein- and RNA-based parts make use of the same endogenous RNA polymerase