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The following variables were included in the analysis: age, gender, type and aetiology of epilepsy [22], duration of epilepsy at the time of ammonia measurement, comorbidities, drug resistance [23], types and frequency of seizures at the time of testing, types, doses, and blood concentrations of ASMs used at the time of measurement, presence of abnormal hepatic or renal function at the time of measurement, clinical symptoms associated with HA, treatment adjustment after HA detection, clinical outcome at least six months after HA detection

No changes in IGF-1 levels

Standards were prepared in matching dilutions of the lysis/extraction buffer in KPE buffer

The inclusion of the microalga scenedesmus sp