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Sikiric et al., 2020a)

NHEK cells were maintained in Keratinocyte Basal Medium 2 (KBM2, PromoCell) supplemented with 0.4% bovine pituitary extract, 0.125 ng ml 1 rh EGF, 5 g ml 1 rh insulin, 0.33 g ml 1 hydrocortisone, 0.39 g ml 1 epinephrine, 10 g ml 1 transferrin and 0.06 mM CaCl 2

Her technique is excellent, and I am beyond happy with my results

Double-click a preset to edit it Double-click a preset to edit it Double-click a preset to edit it Standard insulin syringe Concentration 1.00 mg/mL Draw volume 0.250 mL = 0.25 mg 1.00 mg/mL Units to draw (1 mL syringe) 25 units 1 mL Syringe 25 IU Tips & Notes Timing & Consistency Inject on the same day each week at any time of day, with or without food