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Materials and methods Bioinformatics The A/T content of the sequences coding for the recombinant proteins was assessed with the GC Content Calculator ( The theoretical molecular weight and isoelectric point (pI) of each recombinant protein were calculated with the ExPASy compute pI/Mw tool ( and the charge and number of acidic aminoacids of the proteins were determined using PEPSTATS ( Finally, as codons are unevenly used in different organisms, the codon usage bias between E

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Fragment-based drug design: from then until now, and toward the future