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In this, calvariectomy and/or laparotomy (used to assess intracranial (superior sagittal sinus), portal, inferior caval vein and aortal pressure, and brain swelling and organs lesions and vessels presentation) since used in the therapy to counteract increased intracranial pressure and abdominal compartment syndrome [56,57], did not further contribute to the worst circumstances created by the occlusion of the superior sagittal sinus

After blocking with 20% non-fat milk at room temperature for 1 h, the membranes were incubated with primary antibodies against GPX4 (1:1,000), Nrf2 (1:1,000), HO-1 (1:1,000), HMGB1 (1:1,000) and GAPDH (1:1,000) overnight at 4C

Professional imaging such as MRI or ultrasound can clarify the extent of tissue damage and guide appropriate treatment intensity