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Determination of conventional chemical composition Total alkaloids, water-soluble total sugar, and reducing sugar were determined by the continuous flow method, using the same macromolecular content determination equipment as above, and referring to YC/T 1602002 (Tobacco and tobacco productsDetermination of total alkaloids, 2002), YC/T 2172007 (Tobacco and tobacco productsDetermination of Potassium, 2007) and YC/T 1592019 (Tobacco and tobacco productsDetermination of water soluble sugars, 2019) respectively

This liquid contains nicotine which is also toxic