The researchers showed that blocking glutamine metabolism through JHU083 not only suppressed tumor cell metabolism but also mitigated the tumor microenvironment, which is hostile to the immune response due to its hypoxic, acidic, and nutrient-depleted conditions, unleashing the natural antitumor T cell response
During this process, first-pass metabolism may occur, affecting bioavailability
Quantification of cytoplasmic localization To assess the cytoplasmic localization of fluorescence, we adapted protocols 42 to focus exclusively on intestinal cells
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