The primary antibodies used included anti-ZO-1 (Abcam, ab307799, 1:5000), anti-Occludin (Abcam, ab216327, 1:5000), anti-ACSL4 (Abcam, ab155282, 1:10000), anti-TFR1 (ABclonal, A5865, 1:2000), anti-SLC7 A11 (Abcam, ab175186, 1:5000), anti-GPX4 (Abcam, ab125066, 1:5000), anti-VDAC2 (Proteintech, 116631-AP, 1:5000), anti-VDAC3 (Proteintech, 552601-AP, 1:5000), -tubulin (Proteintech, 100681-AP, 1:5000), and GAPDH (Proteintech, 104941-AP, 1:20000)
"Metabolic studies of a synthetic C-terminal fragment of human growth hormone (AOD9604)." Journal of Endocrinology 166.3 (2000): 75-85
Conversely, under conditions of copper deficiency or FDX1 loss, elesclomol shifts to a protective function, restoring mitochondrial respiration by delivering copper to CCO [57]
Consult a GP if you're considering L-carnitine