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2.8 Assessment of cell cycle Cells were seeded into 12-well plates at optimised densities (HCT116: 150,000 cells/well, SW837: 200,000 cells/well) in cRPMI and allowed to adhere overnight at 37C in 5% CO 2 /95% humidified air

Additionally, individuals taking medications, especially those for diabetes or heart conditions, should consult with a healthcare provider before incorporating ACV into their routine, as it may interact with certain medications

Add 3ml sterile water (bacteriostatic) into each vial 2

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