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In addition to donating an electron during the reduction of hydroperoxides to the respective alcohols (or water in the case of hydrogen peroxide), GSH functions as a co-substrate in the metabolism of xenobiotics catalyzed by glutathione S-transferases

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The proteins were purified from cell culture supernatant using a human albumin affinity column, followed by size exclusion chromatography (SEC), which yielded monomeric fractions that migrated on non-reducing sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels according to their molecular weight (MW) (66.5 kDa) (Fig

From a methodological standpoint, BPC-157 experiments often involve titration across multiple concentrations, time-course sampling, and pairing with imaging or molecular assays