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After collagen polymerization (1 hour at 5% CO 2 and 37C), each well was filled with 73 l of culture media and left overnight in the incubator for cell acclimatization

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When administered to newly hatched chicks, SynComBac10 promoted early colonization of segmented filamentous bacteria, inducing Th17 cell-mediated mucosal immunity and conferring significant resistance to Salmonella challenge, as evidenced by reduced bacterial loads, attenuated systemic inflammation, and improved intestinal barrier integrity