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The mice were randomly grouped and treated with PBS, free OLE, PEG-TECM-C18/OEL, and PEG-TECM-NS/OLE (total OLE dose: 1 mg kg 1 )

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In certain embodiments of the present disclosure, the amount of buffer included in the gel formulations are an amount such that the pH of the gel formulation does not interfere with the auris media or auris interna's natural buffering system, or does not interfere with the natural pH of the endolymph or perilymph, depending on where in the cochlea the otic formulation is targeted