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Research suggested that this lipolysis peptide may potentially facilitate the release of fat from adipose tissue while moderating the accumulation of new fat deposits in laboratory models, without appearing to alter caloric intake or appetite or adversely affect insulin sensitivity in these experimental settings

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RAW 264.7 cells were cultured in complete Dulbeccos modified Eagle medium (DMEM, Gibco) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Avantor Seradigm), 2 mM L-glutamine (Gibco), 1 non-essential amino acids solution (Gibco) and 1 mM sodium pyruvate (Gibco) below 18 passages without exceeding 90% confluency

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