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Sulforaphane and sulforaphane-nitrile metabolism in humans following broccoli sprout consumption: inter-individual variation, association with gut microbiome composition, and differential bioactivity

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To obtain pgfp, a gfp containing fragment was released from pUCG-H [34] via Sma I and Sac I and subcloned into the corresponding Eco RV and Sac I sites of pGEM5zf+ (Promega)

Previous research suggested that TERT might contribute to lifespan extension through oxidative stress modulation and protection from oxidative damage, which is known to contribute to aging [5]