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Isolation of polar metabolites For the metabolomics of tissues, 100200 mg of weighed tissue was homogenized in 1 ml of ice-cold 80% methanol with a QIAGEN Tissue Lyser II (three sets of 2 min on maximum speed, with 2 min of rest on dry ice in between sets)

For instance, some protocols might explore higher doses for short, acute periods, while others favor lower, sustained dosing for chronic conditions