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Protein turnover in growing cultures of Bacillus megaterium

Testing too soon before the full 48-hour window passes

To confirm this, we performed uniformly 13 C-labeled palmitic acid ([U- 13 C]PA) ( m + 16) flux analysis in glucose-deprived cells cultured in delipidated medium, in which PA ( m + 16)-derived acetyl-CoA ( m + 2) enters the TCA cycle and produces [ 13 C]citrate ( m + 2) and [ 13 C]malate ( m + 2) (Supplementary Fig