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Document baseline mood, anxiety levels, sleep quality, cognitive function, and any gastrointestinal symptoms using standardized self-report measures

The right jugular veins of the rats were catheterized with sterile polyurethane Hydrocoat catheters (Access Technologies, Skokie, Ill., USA) under ketamine (Fort Dodge Animal Health, Fort Dodge, Iowa, USA) and isoflurane (Vet One, MWI, Meridian, Id., USA) anesthesia

Single nucleotide polymorphism in the gene leading to an Arg 213 His amino acid substitution is relatively frequent in the Caucasian population (25.436.5%) (Glatt & Meinl, 2004)