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To investigate the influence of a Western diet, 7- to 10-week-old WT and Gpx4 +/IEC mice were fed a Western diet (ssniff, TD88137) with or without 10% fish oil (ssniff supplementation, see Table S2) and compared to mice receiving a low-fat diet (ssniff, CD88137) for 3 months ad libitum with or without -tocopherol supplementation (0.4 mg/ml, Sigma) in drinking water ad libitum