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To further purify FOXO4 FHD and p53 TAD, gel filtration chromatography was conducted using Hi-Load 16/600 superdex 200pg and 75pg (Cytiva) on AKTA pure and AKTA prime systems (Cytiva)

With the aid of solid-state structures, cellular assays and metabolic stability studies in MLM, we demonstrate that the aryl-fused BCHep derivatives exhibit geometrically similar substituent vectors to the parent compound, retain interesting biological activity and display increased metabolic stability relative to their naphthyl counterpart