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To critically appraise mechanisms and levels of evidence ( in vitro, in vivo , clinical) for vitamin E and C, glutathione and its precursors, polyphenols (quercetin, resveratrol, curcumin), Crocus sativus carotenoids (crocin/safranal), and indirect modulators (e.g., omega-3, folinic acid), emphasizing study quality, translational relevance, and limitations

Twenty-four hours before each experiment, media were replaced with 750 L fresh media containing 1 mM HEPES (Gibco/Fisher Scientific, Cat# 15-630-080)

With it, the improvements tend to stick