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In animal models and in-vitro studies, this peptide has been observed to interact with the Nitric Oxide (NO) system and VEGF (Vascular Endothelial Growth Factor) pathways

A study conducted on hypothyroid rats in 2022 found that black seed oil raised T3 levels, and another study showed that a powder of black cumin seed was able to raise T3 levels over a period of eight weeks, while also reducing the synthesis of anti-TPO antibodies

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